Singer Lab

Decoding Memory In Health and Disease

  • Home
  • Research
  • Lab Members
  • Publications
  • Resources
  • News

Multi-neuron intracellular recording in vivo via interacting autopatching robots

January 3, 2018 by Matthew Attokaren

The activities of groups of neurons in a circuit or brain region are important for neuronal computations that contribute to behaviors and disease states. Traditional extracellular recordings have been powerful and scalable, but much less is known about the intracellular processes that lead to spiking activity. We present a robotic system, the multipatcher, capable of automatically obtaining blind whole-cell patch clamp recordings from multiple neurons simultaneously. The multipatcher significantly extends automated patch clamping, or ‘autopatching’, to guide four interacting electrodes in a coordinated fashion, avoiding mechanical coupling in the brain. We demonstrate its performance in the cortex of anesthetized and awake mice. A multipatcher with four electrodes took an average of 10 min to obtain dual or triple recordings in 29% of trials in anesthetized mice, and in 18% of the trials in awake mice, thus illustrating practical yield and throughput to obtain multiple, simultaneous whole-cell recordings in vivo.

 

More info here.

Filed Under: Uncategorized

 

JOIN THE LAB

We are seeking highly motivated and innovative undergraduate and graduate students and post-docs. We take an interdisciplinary approach, therefore individuals with engineering, computational, or neuroscience experience are welcome to contact Dr. Singer to discuss further.  Please include a cover letter and CV or resume with your correspondence.

NEWS

Congratulations to Nuri Jeong for winning a Young Investigator Training Program award for the 10th IBRO World Congress of Neuroscience in Daegu, Korea this year.  Great job, Nuri!  

Annabelle Singer and Dean McLaughlin discuss memory, Alzheimer’s, and our research in the Dean’s podcast, The Uncommon Engineer:           Listen to more episodes of the Dean’s Uncommon Engineer series here:

As a National Academy of Sciences Kavli Fellow, Dr. Singer attended the 18th Chinese-American Kavli Frontiers of Science symposium in Nanjing, China. The Kavli Frontiers of Science symposium series (KFoS) is the Academy’s premiere activity for distinguished young scientists.  Kavli Frontiers of Science symposia bring together outstanding young scientists to discuss exciting advances and opportunities in a […]

Congratulations to Steph Prince for being selected for the SFN Trainee Professional Development Award.  More details here.  

The Singer lab was awarded a 5-year grant from the NIH National Institute for Neurological Disorders and Stroke (NINDS).  We are grateful to the NIH for it’s continued support of basic research.

Copyright © 2019 · Beautiful Pro Theme on Genesis Framework · WordPress · Log in